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Bild von Tumisu auf Pixabay
06.12.2023

Antimicrobial coating: Bacteria, stay out!

Hospital germs and pathogens are not always transmitted directly from person to person. They can also spread via germ-contaminated surfaces and objects. Empa researchers, together with the chemical company BASF, Spiez Laboratory and the Technical University of Berlin, have now developed coated textiles that inhibit or even kill pathogens. They could be used as antimicrobial curtains in hospitals in the future.

Hospital germs and pathogens are not always transmitted directly from person to person. They can also spread via germ-contaminated surfaces and objects. Empa researchers, together with the chemical company BASF, Spiez Laboratory and the Technical University of Berlin, have now developed coated textiles that inhibit or even kill pathogens. They could be used as antimicrobial curtains in hospitals in the future.

Countless times a day, patients, visitors and medical staff in hospitals touch surfaces of all kinds. Door handles, railings or elevator buttons can serve as transport vehicles for pathogens such as hospital germs or viruses. Smooth surfaces are comparatively easy to clean after contamination. With porous structures such as textiles, however, this is not that simple. Empa researchers have solved this problem together with experts from BASF, Spiez Laboratory and the Technical University of Berlin: A coating process can now be used to treat fabrics in such a way that bacterial and viral pathogens are killed or inhibited in their growth. In hospitals, the coated textiles could be used in future as antimicrobial curtains between patient beds, for example.

Active for months
"We were looking for a process that reliably prevents germs from contaminating textiles that come into contact with a large number of people during use," explains Peter Wick from Empa's Particles-Biology Interactions laboratory in St. Gallen. In this way, chains of infection could be interrupted in which multi-resistant bacteria or viral pathogens, for example, settle on hospital curtains and can then be spread by people.

The researchers ultimately developed a coating process in which a benzalkonium chloride-containing disinfectant was evenly applied to hospital curtains. After optimizing parameters such as concentration, exposure time, processing pressure and drying, the coating adhered stably to the textile surface. But did the coated textiles also exhibit a germicidal effect? This was to be shown by analyzing the antimicrobial activity of the first fabric samples.

"The results of the laboratory tests were very encouraging," says Wick. When the bacterial cultures of some typical problem germs were incubated with the fabric samples, the coated textiles inhibited the growth of staphylococci and pseudomonas bacteria, for example. "The hospital germs were significantly reduced or even killed after just ten minutes of exposure," says the Empa researcher. Moreover, the coating was also active against viral pathogens: Over 99 percent of the viruses were killed by the coated fabric samples.

Another advantage: The coatings remained effective even after several months of storage. This allows production in stock. With the new process, other textiles, filters or cleaning utensils could also be quickly and safely treated with antimicrobials in the future, for example in the event of an epidemic, emphasizes Empa researcher Wick.

Source:

EMPA

(c) Empa
05.04.2022

In the heat of the wound: Smart bandage

A bandage that releases medication as soon as an infection starts in a wound could treat injuries more efficiently. Empa researchers are currently working on polymer fibers that soften as soon as the environment heats up due to an infection, thereby releasing antimicrobial drugs.

It is not possible to tell from the outside whether a wound will heal without problems under the dressing or whether bacteria will penetrate the injured tissue and ignite an inflammation. To be on the safe side, disinfectant ointments or antibiotics are applied to the wound before the dressing is applied. However, these preventive measures are not necessary in every case. Thus, medications are wasted and wounds are over-treated.

A bandage that releases medication as soon as an infection starts in a wound could treat injuries more efficiently. Empa researchers are currently working on polymer fibers that soften as soon as the environment heats up due to an infection, thereby releasing antimicrobial drugs.

It is not possible to tell from the outside whether a wound will heal without problems under the dressing or whether bacteria will penetrate the injured tissue and ignite an inflammation. To be on the safe side, disinfectant ointments or antibiotics are applied to the wound before the dressing is applied. However, these preventive measures are not necessary in every case. Thus, medications are wasted and wounds are over-treated.

Even worse, the wasteful use of antibiotics promotes the emergence of multi-resistant germs, which are an immense problem in global healthcare. Empa researchers at the two Empa laboratories Biointerfaces and Biomimetic Membranes and Textiles in St. Gallen want to change this. They are developing a dressing that autonomously administers antibacterial drugs only when they are really needed.

The idea of the interdisciplinary team led by Qun Ren and Fei Pan: The dressing should be "loaded" with drugs and react to environmental stimuli. "In this way, wounds could be treated as needed at exactly the right moment," explains Fei Pan. As an environmental stimulus, the team chose a well-known effect: the rise in temperature in an infected, inflamed wound.

Now the team had to design a material that would react appropriately to this increase in temperature. For this purpose, a skin-compatible polymer composite was developed made of several components: acrylic glass (polymethyl methacrylate, or PMMA), which is used, for example, for eyeglass lenses and in the textile industry, and Eudragit, a biocompatible polymer mixture that is used, for example, to coat pills. Electrospinning was used to process the polymer mixture into a fine membrane of nanofibers. Finally, octenidine was encapsulated in the nanofibers as a medically active component. Octenidine is a disinfectant that acts quickly against bacteria, fungi and some viruses. In healthcare, it can be used on the skin, on mucous membranes and for wound disinfection.

Signs of inflammation as triggers
As early as in the ancient world, the Greek physician Galen described the signs of inflammation. The five Latin terms are still valid today: dolor (pain), calor (heat), rubor (redness), tumor (swelling) and functio laesa (impaired function) stand for the classic indications of inflammation. In an infected skin wound, local warmth can be as high as five degrees. This temperature difference can be used as a trigger: Suitable materials change their consistency in this range and can release therapeutic substances.

Shattering glove
"In order for the membrane to act as a "smart bandage" and actually release the disinfectant when the wound heats up due to an infection, we put together the polymer mixture of PMMA and Eudragit in such a way that we could adjust the glass transition temperature accordingly," says Fei Pan. This is the temperature, at which a polymer changes from a solid consistency to a rubbery, toughened state. Figuratively, the effect is often described in reverse: If you put a rubber glove in liquid nitrogen at –196 degrees, it changes its consistency and becomes so hard that you can shatter it like glass with one blow.

The desired glass transition temperature of the polymer membrane, on the other hand, was in the range of 37 degrees. When inflammation kicks in and the skin heats up above its normal temperature of 32 to 34 degrees, the polymer changes from its solid to a softer state. In laboratory experiments, the team observed the disinfectant being released from the polymer at 37 degrees – but not at 32 degrees. Another advantage: The process is reversible and can be repeated up to five times, as the process always "switches itself off" when it cools down. Following these promising initial tests, the Empa researchers now want to fine-tune the effect. Instead of a temperature range of four to five degrees, the smart bandage should already switch on and off at smaller temperature differences.

Smart and unsparing
To investigate the efficacy of the nanofiber membranes against wound germs, further laboratory experiments are now in the pipeline. Team leader Qun Ren has long been concerned with germs that nestle in the interface between surfaces and the environment, such as on a skin wound. "In this biological setting, a kind of no man's land between the body and the dressing material, bacteria find a perfect biological niche," says the Empa researcher. Infectious agents such as staphylococci or Pseudomonas bacteria can cause severe wound healing disorders. It was precisely these wound germs that the team allowed to become acquainted with the smart dressing in the Petri dish. And indeed: The number of bacteria was reduced roughly 1000-fold when octenidine was released from the smart dressing. "With octenidine, we have achieved a proof of principle for controlled drug release by an external stimulus," said Qun Ren. In future, she said, the technology could be applied to other types of drugs, increasing the efficiency and precision in their dosage.

The smart dressing
Empa researchers are working in interdisciplinary teams on various approaches to improve medical wound treatment. For example, liquid sensors on the outside of the dressing are to make it visible when a wound is healing poorly by changing their color. Critical glucose and pH values serve as biomarkers.

To enable bacterial infections to be contained directly in the wound, the researchers are also working on a polymer foam loaded with anti-inflammatory substances and on a skin-friendly membrane made of plant material. The cellulose membrane is equipped with antimicrobial protein elements and kills bacteria extremely efficiently in laboratory tests.

Moreover, digitalization can achieve more economical and efficient dosages in wound care: Empa researchers are developing digital twins of the skin that allow control and prediction of the course of a therapy using real-time modeling.

Further information:
Prof. Dr. Katharina
Maniura Biointerfaces
Phone +41 58 765 74 47
Katharina.Maniura@empa.ch

Prof. Dr. René Rossi
Biomimetic Membranes and Textiles
Phone +41 58 765 77 65
Rene.rossi@empa.ch

Source:

EMPA, Andrea Six

Bild: Christine Sponchia auf Pixabay
05.05.2020

COVID-19: German Down and Feather Industry exemplary in Terms of Hygiene

  • Update on the economic situation of the industry
  • Supply availability secured for the next half-year
  • E-commerce wins in the crisis
  • Sector survey of the Association of the German Down and Feather Industry VDFI and Traumpass e.V. on the occasion of the Corona crisis

The corona pandemic not only poses great challenges for each and every one of us, but also for our businesses as an industry. The decisions of the Federal Government and the federal states to close down shops and restrict freedom of mobility have hit the predominantly medium-sized down and feather industry hard, not only on the sales side, but also through their trading partners. The declining demand was compounded by the industry's international dependencies within the supply chains, since the filling material, the covers and the packaging materials are largely imported.

  • Update on the economic situation of the industry
  • Supply availability secured for the next half-year
  • E-commerce wins in the crisis
  • Sector survey of the Association of the German Down and Feather Industry VDFI and Traumpass e.V. on the occasion of the Corona crisis

The corona pandemic not only poses great challenges for each and every one of us, but also for our businesses as an industry. The decisions of the Federal Government and the federal states to close down shops and restrict freedom of mobility have hit the predominantly medium-sized down and feather industry hard, not only on the sales side, but also through their trading partners. The declining demand was compounded by the industry's international dependencies within the supply chains, since the filling material, the covers and the packaging materials are largely imported. The closure of the borders within Europe and the overall tense global logistics situation contributed significantly to the worsening of the situation.
 
In the past few days, the federal and state governments have started cautious attempts to ease the contact bans and to revive the economy with a sense of proportion. The opening of shops and the continuation of business activities prompted the down and feather associations to question the status quo of the sector with an extensive survey.

Although the companies named significant losses in their turnover, they currently still got off relatively lightly compared with other sectors. Two thirds of the companies stated that they had suffered up to 25% sales losses due to the corona crisis. Approximately 17% reported a decline of up to 50%, the ones with same percentage were much harder hit with a decline of up to 75%.

On the occasion of Heimtextil in January 2020, the association's sector statement had described the position of the specialised trade as still stable from the bedding manufacturers' point of view: By expanding the range of services, such as cleaning down and feathers or refilling existing bedding, the local store was increasingly becoming a point of contact for consumers. Supporting the advisory competence and the deployment of sleep experts made an impact. This picture has changed significantly as a result of the contact ban:

The clear loser in terms of demand on the various sales channels, caused by the shop closures, was the traditional retail: 92% of the surveyed manufacturing companies registered declining demand for the retail trade, 90% for the furniture trade and 80% for the specialty stores. Even for the discounters, whose opening hours were not affected, 33% noted a drop in demand; 44% estimated the demand situation as unchanged. The winner in the crisis was the e-commerce, although perhaps to a lesser extent than expected: 45% of the German down and feather producers recorded an increase in demand, 36% estimated the level as unchanged.

In terms of supply capability, the industry in Germany considers itself as well positioned: Two thirds see no bottlenecks for the fulfilment of closed contracts within the next three months, and the majority also offers free capacities beyond that. And 55% guarantee this ability to deliver even for the next six months, including the satisfaction of additional requirements.

While 2019 was a year of consolidation for the German down and feather sector and, after difficult months with sharply increased raw material prices, a calming down on a high level prevailed, good results from the previous year were maintained and, in some cases, even increased, the assessment for 2020 is much more pessimistic.

As far as the price situation for the coming autumn/winter season is concerned, member companies were correspondingly cautious in their forecasts.    
The unresolved and in some cases very fragile situation in the supplier countries currently not allows to make reliable statements. At the earliest in late summer, a well-founded opinion can be obtained. Especially since 45% of the companies expect to be confronted with requests for price reductions.
 
The down and feather industry is a professional when it comes to hygiene. The highest purity requirements are placed on down and feathers: Before being used as filling material, they must be cleaned by thorough water washing and dried at a temperature of at least 100°C, usually higher. This ensures that bedding meets the hygiene requirements of European Standard EN 12935 with reliably killing bacteria, viruses and other germs. In addition to the applicable standards, the companies have taken additional precautions to protect employees, trade partners and consumers.

In addition to the intensive workplace and hand disinfection, which all manufacturers have increased, the companies focused particularly on the equalization of the workforce. 73% designed home office workplaces for employees outside production, 45% introduced strict shift separation, two thirds shifted working hours and changed the use of common rooms in order to have as few employees as possible in the company at the same time and thus minimized the risk of infection. At the same time, many companies started the production of mouth and nose masks and made their sewing facilities available for this purpose. A list of the manufacturing companies is available from the Association of the German Down and Feather Industry VDFI e.V.

With the start of the lockdown, the German government had announced extensive financial aid, which focused on bridging payments for small businesses, granting loans, short-time work and tax deferrals. Improvements had to be made for small and medium-sized enterprises. In the recent weeks, the sector has not primarily addressed the question for bridging loans nor reducing the interest burden; emergency aid in the form of financial injections and non-repayable grants was also of interest only to one third. The majority of the association members concentrated on measures to secure liquidity (45%), tax deferrals and the suspension of advance payments of VAT (73%) as well as the changeover to short-time work (73%) and the accelerated processing and granting of short-time work compensation (45%). Also, two thirds requested binding statements on the easing of the contact ban and on the economic upturn.

An update on the industry survey is planned for late summer 2020.

Source:

VDFI e.V. / Traumpass e.V.